UQCRFS1 antibody [N1C3], 0.32 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 7386 |
| Protein Name / Synonyms | UQCRFS1 |
| Concentration (lot specific) | 0.32 mg/ml |
| Recommended Applications | Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
RIP1 antibody, RIS1 antibody, RISP antibody, UQCR5 antibody, UQCRFS1 antibody, cytochrome b-c1 complex subunit 5 antibody, "cytochrome b-c1 complex subunit Rieske, mitochondrial antibody", complex III subunit 5 antibody, ubiquinol-cytochrome c reductase iron-sulfur subunit antibody, Rieske iron-sulfur protein antibody, "ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
30 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 274 of UQCRFS1 (Uniprot ID# P47985)
Applications
Applications Tested
Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
UQCRFS1 antibody [N1C3] detects UQCRFS1 protein at mitochondria by immunofluorescent analysis.
Sample: A549 cells were fixed in 2% paraformaldehyde/culture medium at 37? for 30 min.
Green: UQCRFS1 protein stained by UQCRFS1 antibody [N1C3] (107-10320) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 ?m.
Sample: A549 cells were fixed in 2% paraformaldehyde/culture medium at 37? for 30 min.
Green: UQCRFS1 protein stained by UQCRFS1 antibody [N1C3] (107-10320) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 ?m.
UQCRFS1 antibody [N1C3] detects UQCRFS1 protein at mitochondria by immunofluorescent analysis.
Sample: H1299 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: UQCRFS1 protein stained by UQCRFS1 antibody [N1C3] (107-10320) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample: H1299 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: UQCRFS1 protein stained by UQCRFS1 antibody [N1C3] (107-10320) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
107-10320 diluted at 1:1000
A: Mouse brain
12% SDS PAGE
107-10320 diluted at 1:1000
Sample(30 ug whole cell lysate)
A: Raji
12% SDS PAGE
107-10320 diluted at 1:500
A: Raji
12% SDS PAGE
107-10320 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.