USF1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P22415 |
| Gene Id | 7391 |
| Gene Symbols | USF1 |
| Protein Name / Synonyms | USF1, FCHL, FCHL1, HYPLIP1, MLTF, MLTFI, UEF, bHLHb11, upstream stimulatory factor 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. This gene has been linked to familial combined hyperlipidemia (FCHL). Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been defined on chromosome 21.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
27kDa/33kDa
Observed MW
41kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human USF1 (NP_009053.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of mouse kidney, using USF1 antibody (144-60393) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human kidney cancer using USF1 antibody (144-60393) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using USF1 antibody (144-60393) at dilution of 1:200 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.