Rabbit Anti-USF1 (Center) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P22415 |
| Gene Id | 7391 |
| Gene Symbols | USF1 |
| Protein Name / Synonyms | USF1 (Center) |
| Clonality | Polyclonal |
| Preparation | This USF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 174-201 amino acids from the Central region of human USF1. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, FC, IF, IHC-P |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. This gene has been linked to familial combined hyperlipidemia (FCHL). Two transcript variants encoding distinct isoforms have been identified for this gene.
Specificity
Predicted Reactivity
Bovine
Other Names
USF1; BHLHB11; USF; Upstream stimulatory factor 1; Class B basic helix-loop-helix protein 11; Major late transcription factor 1
NCBI Accession #
NP_001263302.1;NP_009053.1;NP_996888.1
Other Accession #
Q61069; Q6XBT4; NP_009053.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
33538
Recommended Dilutions
WB: 1:1000; FC: 1:10-50; IF: 1:10-50; IHC: 1:10-50
 | USF1 Antibody (Center) (Cat# 102-10926) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the USF1 antibody detected the USF1 protein (arrow). |
 | USF1 Antibody (Center) (Cat# 102-10926) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Fluorescent confocal image of SK-BR-3 cell stained with USF1 Antibody (Center)(Cat# 102-10926). SK-BR-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with USF1 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).USF1 immunoreactivity is localized to nucleus significantly. |
 | USF1 Antibody (Center) (Cat# 102-10926)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of USF1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.