USO1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | O60763 |
| Gene Id | 8615 |
| Gene Symbols | USO1 |
| Protein Name / Synonyms | USO1, P115, TAP, VDP, USO1 vesicle transport factor |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
107kDa/109kDa
Observed MW
115kDa
Immunogen
A synthetic peptide of human USO1
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using USO1 antibody (144-02796) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human kidney using USO1 antibody (144-02796) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using USO1 antibody (144-02796) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using USO1 antibody (144-02796) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using USO1 antibody (144-02796) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterine cancer using USO1 antibody (144-02796) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using USO1 antibody (144-02796). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.