This gene complements the ultraviolet sensitivity of xeroderma pigmentosum group C cells and encodes a protein with a C2 domain. The protein activates the Beclin1-PI(3)KC3 complex, promoting autophagy and suppressing the proliferation and tumorigenicity of human colon cancer cells. Chromosomal aberrations involving this gene are associated with left-right axis malformation and mutations in this gene have been associated with colon cancer.
Dilutions
WB 1:500 - 1:2000 IHC 1:50 - 1:100
Format
Calculated MW
35kDa/78kDa
Observed MW
100kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 501-699 of human UVRAG (NP_003360.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using UVRAG antibody (144-08462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human liver injury using UVRAG antibody (144-08462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using UVRAG antibody (144-08462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using UVRAG antibody (144-08462) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.
