Rabbit Anti-V5 Tag Antibody, 400 ul
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Host Species | Rabbit |
| Protein Name / Synonyms | V5 Tag |
| Clonality | Polyclonal |
| Preparation | KLH conjugated synthetic peptide encoding the V5 tag (GKPIPNPLLGLDST) was used as antigen. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The V5 epitope tag is is composed of a 14 residue peptide, GKPIPNPLLGLDST, derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). RayBiotech's anti-V5 tag polyclonal antibody provides a simple solution to detect the expression of a V5-tagged protein in cells.
Specificity
Other Names
Tag from influenza hemagglutinin protein
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Recommended Dilutions
WB: 1:1000
 | Lanes: 1:non-transfected cells; 2:V5-tagged empty plasmid; 3:protein A (V5-tagged); 4:protein B (V5-tagged); 5:protein C (V5-tagged); 6:protein D (V5-tagged); Protocol and data courtesy of Dr. Gustavo Gutierrez, Burnham Institute for Medical Research. |
 | Western blot analysis of anti-V5 Tag Antibody (Cat# 102-10569) in 12tag protein (35ug/lane). 12 Tag (arrow) was detected using the purified Pab. |
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.