Rabbit Anti-WNT10B (Center) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | O00744 |
| Gene Id | 7480 |
| Gene Symbols | WNT10B |
| Protein Name / Synonyms | WNT10B (Center) |
| Clonality | Polyclonal |
| Preparation | This WNT10B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 193-222 amino acids from the Central region of human WNT10B. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Research Area | Wnt-beta-Catenin Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT10B is a member of the WNT family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level.
Specificity
Other Names
WNT10B; WNT12; Protein Wnt-10b; Protein Wnt-12
NCBI Accession #
NP_003385.2
Other Accession #
P48614
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
43000
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; FC: 1:10-50
 | Western blot analysis of WNT10B Antibody (Center) (Cat# 102-16842) in MDA-MB231 cell line lysates (35ug/lane). WNT10B (arrow) was detected using the purified Pab. |
 | Western blot analysis of WNT10B Antibody (Center) (Cat# 102-16842) in mouse liver tissue lysates (35ug/lane). WNT10B (arrow) was detected using the purified Pab. |
 | WNT10B Antibody (Center) (Cat# 102-16842) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WNT10B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | Flow cytometric analysis of MDA-231 cells using WNT10B Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.