The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants.
WB 1:500 - 1:2000
IHC 1:50 - 1:100
Recombinant fusion protein containing a sequence corresponding to amino acids 120-359 of human WNT5B (NP_116031.1).
Western blot analysis of extracts of various cell lines, using WNT5B antibody (144-08313) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Immunohistochemistry of paraffin-embedded mouse lung using WNT5B antibody (144-08313) at dilution of 1:100 (40x lens).
12 months from the date of shipment when stored properly.