XRCC5 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P13010 |
| Gene Id | 7520 |
| Gene Symbols | XRCC5 |
| Protein Name / Synonyms | XRCC5, KARP-1, KARP1, KU80, KUB2, Ku86, NFIV, X-ray repair cross complementing 5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.
Dilutions
WB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:20 - 1:100
IP 1:50 - 1:200
IHC 1:100 - 1:200
IF 1:20 - 1:100
IP 1:50 - 1:200
Format
Calculated MW
82kDa
Observed MW
86kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 543-732 of human XRCC5 (NP_066964.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using XRCC5 antibody (144-05862) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human adenomyosis using XRCC5 Antibody (144-05862) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using XRCC5 Antibody (144-05862) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using XRCC5 Antibody (144-05862) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using XRCC5 Antibody (144-05862) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using XRCC5 Antibody (144-05862) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using XRCC5 Antibody (144-05862) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using XRCC5 Antibody (144-05862) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human adenomyosis using XRCC5 Antibody (144-05862) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using XRCC5 antibody (144-05862). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using XRCC5 antibody (144-05862) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.