ZEB2 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O60315 |
| Gene Id | 9839 |
| Gene Symbols | ZEB2 |
| Protein Name / Synonyms | ZEB2, HSPC082, SIP-1, SIP1, SMADIP1, ZFHX1B, zinc finger E-box-binding homeobox 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a member of the Zfh1 family of 2-handed zinc finger/homeodomain proteins. It is located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Mutations in this gene are associated with Hirschsprung disease/Mowat-Wilson syndrome. Alternatively spliced transcript variants have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
133kDa/136kDa
Observed MW
136kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1050-1214 of human ZEB2 (NP_055610.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ZEB2 antibody (144-05705) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using ZEB2 antibody (144-05705) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using ZEB2 antibody (144-05705) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using ZEB2 antibody (144-05705) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using ZEB2 antibody (144-05705) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using ZEB2 antibody (144-05705) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ZEB2 antibody (144-05705) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.