ZFYVE9 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | O95405 |
| Gene Id | 9372 |
| Gene Symbols | ZFYVE9 |
| Protein Name / Synonyms | ZFYVE9, MADHIP, NSP, PPP1R173, SARA, SMADIP, zinc finger FYVE-type containing 9 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a double zinc finger motif-containing protein that participates in the transforming growth factor-beta (TGFB) signalling pathway. The encoded protein interacts directly with SMAD2 and SMAD3, and recruits SMAD2 to the TGFB receptor. There are multiple pseudogenes for this gene on chromosomes 2, 15, and X. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:200 - 1:2000
IF 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
83kDa/150kDa/156kDa
Observed MW
156kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ZFYVE9 (NP_004790.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ZFYVE9 antibody (144-09041) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.
Immunofluorescence analysis of HeLa cells using ZFYVE9 antibody (144-09041) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.