IL-12 is considered a critical proinflammatory cytokine for autoimmune diseases such as multiple sclerosis and its animal model, experimental autoimmune encephalomyelitis (EAE). IL-12 is a heterodimer composed of a p35 subunit and a common p40 subunit shared by other cytokines. Interleukin (IL)-12p75 is a heterodimeric cytokine composed of the product of two different genes that specify p35 and p40 subunits. Both IL-12 p40(-/-) and p35(-/-) mice fail to produce IL-12 p75 heterodimer. Studies show that a molecule other than IL-12 p75, which uses the p40 subunit, fulfills the functions previously attributed to IL-12 with regard to the development and pathogenesis of this autoimmune disease.

This antibody was selected for its ability to detect mouse IL-12 in ELISA and Array. In ELISA, this antibody shows reactivity with IL-12 p40 and no cross-reactivity with murine IL-10 and other tested proteins.

Lyophilized: store at 4 C. After reconstitution, if not used within a month, aliquot and store at 20 C. Stability: Two years lyophilized, one month in solution at 4°C.