Rat Leptin ELISA


RayBio® Rat Leptin ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

30 pg/ml

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Detection Range
30 pg/ml - 8000 pg/ml
Recommended Dilution (serum & plasma)
3 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data


The Leptin ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, L-Selectin, Lix, MCP-1, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha.
Species Detected
Protein Name & Synonyms
Leptin, Obesity factor
Accession Number
Gene Names
Lep, Ob

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.


The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • LEP

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Rat
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Method of Detection:
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Jain S., Singh S., et al. Effect of L-carnitine and Hoodia gordonii Supplementation on Metabolic Markers and Physical Performance under Short Term Calorie Restriction in Rats. Defence Science Journal, Vol. 66, No. 1, January 2016, pp. 11-18, DOI : 10.14429/dsj.66.9178
    Species: Rat
    Sample Type: Plasma

  2. Jambocus G., Saari N., Ismail A., et al. An Investigation into the Antiobesity Effects of Morinda citrifolia L. Leaf Extract in High Fat Diet Induced Obese Rats Using a (1)H NMR Metabolomics Approach.
    Species: Rat
    Sample Type: Plasma

  3. Govic A., Penman J., Tammer A., Paolini A. Paternal calorie restriction prior to conception alters anxiety-like behavior of the adult rat progeny. Psychoneuroendocrinology. 2016 Feb;64:1-11. doi: 10.1016/j.psyneuen.2015.10.020.
    Species: Rat
    Sample Type: Serum

  4. Thakare M., Surana S. β-Asarone modulate adipokines and attenuates high fat diet-induced metabolic abnormalities in Wistar rats. Pharmacological Research Volume 103, January 2016, Pages 227-235

  5. Saad M., Kamel M., Hanafi M. Modulation of Adipocytokines Production and Serum NEFA Level by Metformin, Glimepiride, and Sitagliptin in HFD/STZ Diabetic Rats. Biochem Res Int. 2015; 2015: 138134. doi: 10.1155/2015/138134
    Species: Rat
    Sample Type: Serum

  6. Tawfik S., Mahmoud B., Saad M. Similar and additive effects of ovariectomy and diabetes on insulin resistance and lipid metabolism. Biochem Res Int. 2015;2015:567945. doi: 10.1155/2015/567945.
    Species: Rat
    Sample Type: Serum

  7. de Castro G., Seminice R., Simoes-Ambrosio L., et al. Dietary Docosahexaenoic Acid and Eicosapentaenoic Acid Influence Liver Triacylglycerol and Insulin Resistance in Rats Fed a High-Fructose Diet. Mar. Drugs 2015, 13(4), 1864-1881; doi:10.3390/md13041864
    Species: Rat
    Sample Type: Serum

  8. Ashwini S., Bobby Z., Joseph M., et al. Insulin plant (Costus pictus) extract improves insulin sensitivity and ameliorates atherogenic dyslipidaemia in fructose induced insulin resistant rats: Molecular mechanism. Journal of Functional Foods. Volume 17, August 2015, Pages 749-760
    Species: Rat
    Sample Type: Plasma

  9. Seif el-Din S., El-Lakkany N., El-Nagger A., et al. Effects of rosuvastatin and/or β-carotene on non-alcoholic fatty liver in rats. Res Pharm Sci. 2015 Jul-Aug; 10(4): 275-287.
    Species: Rat
    Sample Type: Serum

  10. Abdel-Reheim E., et al. Fatty-Sucrosed Diet/Minimal Dose of Streptozotocin Treated Rat: A Novel Model of Gestational Diabetes Mellitus, Metabolic and Inflammatory Insight. J Diabetes Metab 2014, 5:9http://dx.doi.org/10.4172/2155-6156.1000430
    Species: Rat
    Sample Type: Serum

  11. Salem A., et al. Distinct Mechanisms Underlying the Therapeutic Potential of Foeniculum vulgare in Nonalcoholic Fatty Liver Disease - Experimental Study. Int. J. Pharm. Sci. Rev. Res., 28(2), Sept - Oct 2014; Article No. 27, Pages: 150-161
    Species: Human
    Sample Type: Serum

  12. Huang H-Y, Korivi M, Tsai C-H, et al. Supplementation of Lactobacillus plantarum K68 and Fruit-Vegetable Ferment along with High Fat-Fructose Diet Attenuates Metabolic Syndrome in Rats with Insulin Resistance. Evid-Based Compl Alt Med. 2013;2013:943020.
    Species: Rat
    Sample Type: Serum

  13. Huang H-Y, Korivi M, Tsai C-H, et al. Supplementation of Lactobacillus plantarum K68 and Fruit-Vegetable Ferment along with High Fat-Fructose Diet Attenuates Metabolic Syndrome in Rats with Insulin Resistance. Evid-Based Compl Alt Med. 2013;2013:943020.
    Species: Rat
    Sample Type: Serum

  14. Jain S, Singh SN. Metabolic effect of short term administration of Hoodia gordonii, an herbal appetite suppressant. So Afr J Bot. 2013 May;86:51-55.
    Species: Rat
    Sample Type: Plasma

  15. Mohamed SH, Ahmed HH, Farrag ARH, et al. Cynara scolymus for relieving on nonalcoholic steatohapatitis induced in rats.Int J Pharm Pharmaceut Sci. 2013;5(Suppl. 1):57-66.
    Species: Rat
    Sample Type: Serum

  16. Zhang Z., Wang Y., Long Y., Wang L., Li Y., Gao F., Tian H. Alteration of sweet taste in high-fat diet induced obese rats after 4 weeks treatment with exenatide. Peptides 47 (2013) 115-123
    Species: Rat
    Sample Type: Serum

  17. Slobadoa N., Feve B., Thornton S., et al. Fatty Acids Impair Endothelium-Dependent Vasorelaxation: A Link Between Obesity and Arterial Stiffness in Very Old Zucker Rats.
    Species: Rat
    Sample Type: Plasma

  18. Jain S., Verma S., Sing V., Sing S. Effect of Short Term Sibutramine Supplementation on Appetite Suppression and Related Metabolic Responses. Journal of Pharmacy and Nutrition Sciences, 2012, 2, 165-171. DOI: http://dx.doi.org/10.6000/1927-5951.2012.02.02.8
    Species: Rat
    Sample Type: Plasma

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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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