Introduction
RayBiotech is committed to develop a series of phosphorylation antibody arrays. RayBio® Rat Protein Tyrosine Phosphorylation Antibody Array 2 is specifically designed for simultaneous identification of the relative levels of phosphorylation of 500 different rat proteins in cell lysate. By monitoring the changes in protein tyrosine phosphorylation in your experimental model system, you can verify pathway activation in your cell lines without spending excess time and effort performing an analysis of immunoprecipitation and/or Western Blot.
To use the Rat Protein Tyrosine Phosphorylation Antibody Array 2, treated or untreated cell lysate is added into antibody array glass slide wells. The antibody array slide wells are washed, and biotinylated anti-phosphotyrosine antibodies are then used to detect the phosphorylated tyrosines on target proteins. After incubation with a fluorescent dye-conjugated streptavidin (Cy3 equivalent), the slides can then be imaged using a laser scanner, such as the Axon GenePix, using the Cy3 channel.
Product Features
- High sensitivity and specificity
- Low sample volume (10-100 µl per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
Target Names
11b-HSD1, 14-3-3 epsilon, 14-3-3 eta, 14-3-3 sigma, 14-3-3 theta, 4-1BB (TNFRSF9/CD137), A2B5, ACACA, ACTC1, Actin, Activin R2A, ADAM10, ADAMTS-1 (METH1), Adiponectin (ACRP30), aFGF (FGF-1), Agrin, AIF, AK1, ALCAM (CD166), ALK-7 (ACVR1C/Activin A Receptor), Alpha-Actinin 1, Alpha-Galactosidase A (GLA), Alpha-Synuclein, Ameloblastin, AMPK alpha 2, Androgen R, ANGPT1, Annexin A1, Annexin A4, Annexin A7, Annexin V, APE1 (APEX1), APRIL, Arginase 1, ART4, ASAH2, B3GNT2, BAFF, BAK (Bcl2-L-7), BCAM, BCL-10, Bcl-2, Bcl-w, Bcl-xL, beta 2-Microglobulin, beta IG-H3, bFGF, BID, BIK, BLVRB, BMP-2, BMP-7, B-raf, BST1, BTLA (CD272), C4.4A (LYPD3), Cadherin-4, Calcineurin A, Calcineurin B, Carboxylesterase 1 (CES1), Carboxypeptidase A1 (CPA1), Carboxypeptidase B1 (CPB1), Caspr 2, Catalase, Cathepsin B (Pro-Cathepsin B), Cathepsin C, Cathepsin E, Cathepsin L, Cathepsin X, Caveolin-2, CCK-A R, CCL26-Like, CD13, CD14, CD155 (PVR), CD1d1, CD200, CD22, CD226, CD27 (TNFRSF7), CD276, CD300f (CLM-1/IREM-1), CD300LG, CD31, CD34, CD38, CD39L1, CD4, CD47, CD48 (SLAMF2), CD5L, CD6, CD63, CD68, CD79B, CD8 alpha, CD83, CD86 (B7-2), CD93, CDC25B, CDC37, CDH2, CDNF, CHMP2B, Chordin, CIB1 (CIBP), CLEC4A2, CLEC4B2, Clusterin, CNTF, CO5, Coagulation Factor XIV (Protein C), COLEC12, Complexin-2, Contactin-1, Contactin-2 (TAG1), Contactin-4 (BIG-2), Cortactin, CPA2, CRELD1, CRELD2, CrkL, CRP (C-Reactive Protein), CRYAB, CSF1R, CTACK (CCL27), CTGF, CTHRC1, CTLA-4 (CD152), Cubilin, CXCL10, CXCL16, Cyclophilin A, Cyclophilin B, Cystatin C, Cytochrome C, Decorin, DEP-1, DGK-epsilon, DHFR, DKK-3, DLL1, DOK7, DYRK2, DYRK3, E-Cadherin, ECM1, EEA1, EFNA1, EFNB1, EGF, EGFR, eIF5A, EMP, ENO1, Eotaxin-1 (CCL11), EphA5, EphB1, EphB6, Ephrin-A2, Ephrin-B2, ER alpha (NR3A1), ErbB2, ErbB3, Erythropoietin, Ets-1, Ezrin, F2, F3, FABP1, FABP2, FABP3, FABP4 (A-FABP), FABP5 (E-FABP), FAK, FCAR (CD89), FCGR1, FETUB, FGF-12, FGF-21, FGFR4, Fgr, Fibromodulin, FKBP12, FKBP12.6, FKBP13, FKBP25, FKBP51, FKBP52, FLIP, FLT1, Flt-3 Ligand, Follistatin, FOLR1, FRK, FRS2, GABRA4, GAD1, Galectin-1, Galectin-3, Galectin-4, GAPDH, Gas 1, GDF-3, GDF-8 (Myostatin), GDNF, GFAP, GFRA1, GFRA2, GFRA3, GGT1, GIT1, GITR (TNFRSF18), GLG1, GLRX3, Glyoxalase 1, Glyoxalase 2, Glypican 3, gp130 (sgp130), GPT, Gpt2, GPX1, GPX3, Granzyme B, GRB2, GRIN2A, GRK1, GRK2, GRK5, GRO alpha (CXCL1), Growth Hormone, GRP75, HAAO, HABP1, HGF, HIF-1 alpha, HO-2, HPRG, HPX, HSP20, HSP27, HSP40, HSP60, HSP70, HSP90, HSPA8, HSPH1, HTRA2, Human Amyloid-like protein 1 (APLP-1), Iduronate 2-Sulfatase (IDS), IFN alpha, IFN alpha 5, IFN-gamma, IFN-gamma R2, IGF-1, IGFBP-5, IGSF4B (SynCAM3/CADM3), IGSF8 (CD316), IkB-beta, IKK, IL-1 beta (IL-1 F2), IL-1 R1, IL-1 R2, IL-1 Ra (IL-1 F3), IL-10, IL-11 R alpha, IL-12 p70, IL-13 R alpha 2, IL-15 R alpha, IL-17 RC, IL-18, IL-18 BPc, IL-2, IL-2 R alpha, IL-2 R gamma (Common gamma Chain), IL-21, IL-22, IL-23 p19, IL-31, IL-4, IL-4 R, IL-6, IL-7, IL-7 R alpha (CD127), IL-9, IL-9 R (CD129), ILK, ILKAP, IMPDH1, IPP2, Islet-1, Jagged 1, JAM-A (CD321/F11R), JAM-C (CD323), JNK1, JNK2 (MAPK9 isoform alpha-1), KDR, Keap1, Kininogen (Kininostatin), KIRREL3 (NEPH2), KLKB1, Lactoferrin, LAIR1, LAR, LAYN, LDHA, Legumain, Leptin, Leptin Receptor, LIF, LIFR, LILRA5 (ILT-11/CD85f), LILRC2, Lipocalin-2 (NGAL), LMW-PTP (ACP-1), LPHN3, LRP-4, Lymphotoxin beta R (TNF RIII), Lyn, MAG, Matrilin-3, MBL-2, MCAM (CD146), MCP-3 (MARC/CCL7), MEK2, MIF, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-3 beta (CCL19), MKK6 (MEK6), MMP-2, MMP-8, MMP-9, MOG, MP1, MST1, Myeloperoxidase, NCAM-1 (CD56), NCR3, NEDD4, NEDD8, Nephrin, Nestin, Netrin-1, Neurexophilin-1, Neuritin, Neurocan, Neurofascin, Neurogranin, Neuroligin-1, Neuroligin-2, Neuropilin-1, Neuroplastin 65, NFATC3, NF-L, NM23-H1/H2, nNOS, NNUP85, Noggin, Nogo-A, Nope, Notch-1, Notch-2, Notch-3, NPC2, NRAGE, Nrf2, NRXN1 beta, Olfactomedin-1, OLR1, Osteocalcin, OX40 (TNFRSF4), p27/Kip1 (CDKN1B), p38 gamma, p53, PAK1, PAK7, Pax7, P-Cadherin, PCDH-17, PCK1, PDGF R alpha, PDGF-BB, Pentraxin-2, Peroxiredoxin 6, PFKM, PGC, PI3-Kinase p55-gamma, pIgR, PIM2, PKA C a/b, PKC, PKC a, PKC i/l/z, PKM2, PLAUR, Plexin A4, PON3, POR, PP2A CS, PP2C alpha, PPA1, PPP2R4, PRDX 2, PRDX1, PRDX4, Pref-1 (DLK-1/FA1), PRL-3, PRL8A4, PROCR, Prolactin, Properdin, PSAP, PSMA1, PSMA2, PTK7, PTP1B, PVRL2, RalA, RALT, RANTES (CCL5), RBBP4, RBP4, Reg III, Reg3B, Renin 1, RHD, ROBO1, ROCK2, Secretagogin, Semaphorin 4C, Semaphorin 4D, Semaphorin 5A, Semaphorin 7A, Serpin A1, Serpin E1, Serpin F1, Serpin F2 (Alpha-2-Antiplasmin), SH2B1, SHIP2, SHP-1, SIGNR1 (CD209b), SIRP alpha, SLAMF1, SLC4A1, Slit3, Smad 3, Smad 7, SMC1, Sortilin, SOST, SOX1, SOX10, SOX2, SP-D, Src, STAT3, Syndecan-1, Syndecan-2, Syntaxin 1A, TAFA5, Talin1, TCK-1 (CXCL7), TC-PTP, TDP-43, TGF beta R3, THBD, Thioredoxin-2, Tie-2, TIM-1 (KIM-1), TNF alpha, TNF-R1, TNFRSF11A, TNFSF9, Tollip, TPT1, TRAF-2, TRAF-3 Isoform 2, Transferrin, Transgelin, Transglutaminase 2 (TGM2), TREM-1, TRHDE, TrkA, TRKB, TRKC, TWEAK R (TNFRSF12), UCH-L1, UCH-L3, Unc5h1, Unc5h2, VAMP-2, VHR, Vinculin, VSIG1, WFDC2, Wnt5a, XIAP, XPNPEP2, Zyxin
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Rat Tyrosine Phosphorylation Antibody Array G2 Slide(s)
- 2X Cell Lysis Buffer
- Protease Inhibitor Cocktail
- Phosphatase Inhibitor Cocktail Set II
- Blocking Buffer
- 20X Wash Buffer I
- 20X Wash Buffer II
- Biotin-Conjugated Anti-phosphotyrosine
- Fluorescent dye-Conjugated Streptavidin
- Wash Buffer III
- Adhesive film
Other Materials Required
- Shaker
- Laser scanner for fluorescence detection
- Aluminum foil
- Distilled water
- Plastic box
- 50 ml Centrifuge tube
- Isopropanol (2-propanol)
Protocol Outline
- Dry the Glass Slide
- Blocking and Incubation
- Fluorescence Detection
Storage/Stability
Upon receipt, the kit should be stored at -20°C to -80°C. Please use within 6 months from the date of shipment. After initial use, the 2X Cell Lysis Buffer, Blocking Buffer, 20X Wash Buffer I, 20X Wash Buffer II, Biotin-Conjugated Anti-phosphotyrosine and Fluorescent dye-Conjugated Streptavidin (Cy3 equivalent) should be stored at 4°C to avoid repeated freeze-thaw cycles. The Array I Glass Slide, Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail Set II should be kept at -20°C to -80°C. Use within 3 months after initial use.