Introduction
RayBiotech is committed to develop a series of phosphorylation antibody arrays. RayBio® Rat Protein Tyrosine Phosphorylation Antibody Array 3 is specifically designed for simultaneous identification of the relative levels of phosphorylation of 500 different rat proteins in cell lysate. By monitoring the changes in protein tyrosine phosphorylation in your experimental model system, you can verify pathway activation in your cell lines without spending excess time and effort performing an analysis of immunoprecipitation and/or Western Blot.
To use the Rat Protein Tyrosine Phosphorylation Antibody Array 3, treated or untreated cell lysate is added into antibody array glass slide wells. The antibody array slide wells are washed, and biotinylated anti-phosphotyrosine antibodies are then used to detect the phosphorylated tyrosines on target proteins. After incubation with a fluorescent dye-conjugated streptavidin (Cy3 equivalent), the slides can then be imaged using a laser scanner, such as the Axon GenePix, using the Cy3 channel.
Product Features
- High sensitivity and specificity
- Low sample volume (10-100 µl per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
Target Names
Alpha-2 Macroglobulin, AARE, ABCF1, ACAT1, Activin A, ADAMTS10, ADAMTS15, ADAMTSL2, Aggrecan, AHCY, Fetuin A, Akt2, Albumin, AMPKa1, ANGPTL2, ANGPTL3, ANKRD9, ANXA6, APBA2, ApoA, ApoA2, ApoB, ApoE, ARHGAP1, ATG5, ATPG, B3GAT1, B4GalT1, CD80, B7-H2, BAFF R, bax, BDNF, beta-NGF, BLAME, BLMH, BMP-1, BMP-15, BMP-9, BNIP2, BOLA2, Betacellulin, BTF3, C1q, C1s, C3a, C5a, CA1, CA2, CA3, Calbindin D, Cardiotrophin-1, Cathepsin A, CCL28, CCR3, CCR4, CCT3, CD133, CD23, CD24, CD2AP, CD30, CD40 Ligand, CD9, CD90, CDC14, CFH, CFI, CFL1, CGA, CHCHD3, Chemerin, CHGB, Chk1, Chymase, CINC-2, CINC-3, Cingulin, CIP29, Claudin-3, Claudin-4, CNPY2, CNTFR, COL19A1, COTL1, CPE, CRADD, CREB, CRF21, CRHBP, CrkRS, CRTAC1, CRTAM, CRTH-2, Cryptic, CSE1L, CSK, CTNND1, CXCR2, CXCR4, CXCR7, Cyclin D1, Cyclophilin F, Cystatin A, Cystatin B, Cystatin D, Cystatin E, Cystatin S, DAK, DCI, DCXR, DLL4, DMGDH, DSCAM, DSG1, EDA-A2, EDAR, eEF2, EG-VEGF, eIF4E, EMAP-II, Endothelin, Eotaxin-2, EphA1, EphA2, EPHX2, Epiregulin, ERRa, E-Selectin, EVC2, Factor IX, Factor V, Factor VII, Factor XII, FAM3C, Fas, Fas Ligand, FGF-11, FGF-20, FGF-23, FGF-9, FGF-BP, FGFR1, FGFR2, FGFR5, FGG, FH, Fibronectin, Ficolin-2, FLG2, FOXN3, Fractalkine, Frizzled-1, Frizzled-4, Frizzled-5, Frizzled-6, Frizzled-7, Follistatin-like 1, Galanin, GASP-1, GASP-2, GCSF R, GDF-15, GDF-5, GFRA4, Growth Hormone R, GKN1, GLI-2, GLIPR2, Glut1, Glut2, Glut4, Glut5, GM2A, GM-CSF, GP2, gp340, GPD1, GPR-39, Granzyme A, Granzyme M, GRHPR, GRP, GSK-3 beta, GSN, GSR, GSTM1, GSTO1, GULP1, HAI-1, Haptoglobin, HB-EGF, HEG1, Hepassocin, HEXB, HGFA, Histone H2AY, hnRNPL, Hoxb3, HOXD11, HSP10, HSP47, HTRA1, HVEM, ICAM-1, ICAM-2, IDE, IFN-beta, IFNGR1, IGFBP-2, IGSF4C, IL-1 alpha, IL-38, IL-36 Ra, IL-36 alpha, IL-36 gamma, IL-1 R4, IL-1 R6, IL-11, IL-12 p40, IL-12 RB1, IL-13, IL-15, IL-16, IL-17A, IL-17C, IL-17D, IL-19, IL-2 R beta, IL-24, IL-27, IL-28B, IL-3, IL-3 R beta, IL-5, IMP2, INSL3, Inuslin, I-TAC, Jak2, Kallikrein 10, Kallikrein 11, Kallikrein 5, Kallikrein 6, KIF5B, LAMA5, LAMP, LASP1, LBP, Lefty-1, Lefty-A, LHPP, LIX, LPS, LRG1, LRP-6, L-Selectin, LUZP1, Lymphotactin, MAdCAM-1, MAN1, Mcl-1, MCP-1, MCP-5, MDC, MEP1A, Mesothelin, MICB, MIP-3 alpha, MIS RII, Mitofusin 2, MKK3, MKK4, MMP-10, MMP-13, MMP-16, MMP-7, MRP 1, Multimerin 2, MuSK, MyBPC3, NACA1, NADK, NAGPA, NAPRT1, NeuroD1, Neurolysin, Neuropilin-2, Neurturin, Nidogen-1, Nidogen-2, NIT2, NNT, NOV, NPB, NPTXR, NR3C3, Nrf1, OCT3/4, Orexin A, OSCAR, Oncostatin M, Osteoactivin, Osteoadherin, Osteoprotegerin, p130Cas, p21, P4HB, PAPP-A, PCAP, PCPE-1, PD-1, PD-ECGF, PDGF-AA, PDGF-C, PDGF-D, PDGF R beta, PDLIM5, PDZD2, PENK, Pentraxin-3, Perilipin-3, Peroxiredoxin-3, Platelet Factor 4, PFAS, PFDN6, PHGDH, Piccolo, PIK3R2, PINCH1, PIP4K2A, PLA2G1B, PLD4, Plexin B2, PLGF-2, PLS3, PNP, POMC, PON1, PP, PPP1CC, PRAT4B, PRELP, Prolactin R, ProSAAS, Prostasin, Protein Z, Prouroguanylin, PRR4, PRRC2A, PRTN3, P-Selectin, PSMB1, PSMD2, PSMD9, PSME1, PTHLP, PTMA, PYY, QARS, Quiescin Q6, RAGE, Ras, RELM beta, Resistin, REV3L, RHEB, RNASE6, ROBO4, ROR1, RP1, RPL12, RPL23A, RPLP0, RPS13, RPS14, RPS15A, RPS23, RPS3A, RPS5, RPS8, RPS9, RREB1, RSF1, RUSC1, S100A10, S100A11, S100A9, S100 B, SBP-1, SCF, SCF R, SDF4, Septin-7, SERBP1, Serpin A3, Serpin A5, Serpin B5, Serpin C1, SET, sFRP-4, SH3BGRL3, SHBG, SHOX, Siglec-1, SLC38A10, SLITRK1, ALK-1, SLURP1, Smad 1, Smad 4, Smad 5, Smad 8, Somatostatin, SOX5, SPARC, SPINK7, SPTBN5, SSTR2, STXBP2, SVEP1, SYK, SYN1, TACI, TAGLN2, TALDO, TALDO1, Talin-2, TARC, TARS, I-309, Tcf20, TCP1, TDIF2, TECK, Tenascin X, TFF1, TFF2, TGF beta 1, TGF beta 2, TGF beta 3, TGF-beta R1, TGF-beta R2, TIMP-1, TIMP-2, Titin, TK1, TLR1, TLR3, TLR4, TMEFF1, TMEFF2, TMEM223, TOMM70A, TPIS, Tripeptidyl-peptidase I, TRADD, TRAIL R2, TRAM, TRIM14, Tropomyosin 3, TRRAP, Trypsinogen-2, TSLP, Thrombospondin 1, Thrombospondin-2, TSP-4, TTF1, TUBA6, TWF2, TXNDC15, TXNDC5, TYRO10, UBC9, Ubiquitin+1, Ubiquitin+1, UNC45A, UNC5H4, uPA, UROC1, USP2, Uteroglobin, VAP-1, VAP-A, VARS, VDAC1, VEGF-A, VEGF-B, VEGF-C, VEGFR3, VILIP3, Visfatin, Vitronectin, WARS, WISP-1, WISP-2, XPD, XPG, YY1, ZC3H4
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Rat Tyrosine Phosphorylation Antibody Array G3 Slide(s)
- 2X Cell Lysis Buffer
- Protease Inhibitor Cocktail
- Phosphatase Inhibitor Cocktail Set II
- Blocking Buffer
- 20X Wash Buffer I
- 20X Wash Buffer II
- Biotin-Conjugated Anti-phosphotyrosine
- Fluorescent dye-Conjugated Streptavidin
- Wash Buffer III
- Adhesive film
Other Materials Required
- Shaker
- Laser scanner for fluorescence detection
- Aluminum foil
- Distilled water
- Plastic box
- 50 ml Centrifuge tube
- Isopropanol (2-propanol)
Protocol Outline
- Dry the Glass Slide
- Blocking and Incubation
- Fluorescence Detection
Storage/Stability
Upon receipt, the kit should be stored at -20°C to -80°C. Please use within 6 months from the date of shipment. After initial use, the 2X Cell Lysis Buffer, Blocking Buffer, 20X Wash Buffer I, 20X Wash Buffer II, Biotin-Conjugated Anti-phosphotyrosine and Fluorescent dye-Conjugated Streptavidin (Cy3 equivalent) should be stored at 4°C to avoid repeated freeze-thaw cycles. The Array I Glass Slide, Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail Set II should be kept at -20°C to -80°C. Use within 3 months after initial use.