Introduction
RayBiotech is committed to develop a series of phosphorylation antibody arrays. RayBio® Rat Protein Tyrosine Phosphorylation Antibody Array 4 is specifically designed for simultaneous identification of the relative levels of phosphorylation of 500 different rat proteins in cell lysate. By monitoring the changes in protein tyrosine phosphorylation in your experimental model system, you can verify pathway activation in your cell lines without spending excess time and effort performing an analysis of immunoprecipitation and/or Western Blot.
To use the Rat Protein Tyrosine Phosphorylation Antibody Array 4, treated or untreated cell lysate is added into antibody array glass slide wells. The antibody array slide wells are washed, and biotinylated anti-phosphotyrosine antibodies are then used to detect the phosphorylated tyrosines on target proteins. After incubation with a fluorescent dye-conjugated streptavidin (Cy3 equivalent), the slides can then be imaged using a laser scanner, such as the Axon GenePix, using the Cy3 channel.
Product Features
- High sensitivity and specificity
- Low sample volume (10-100 µl per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
Target Names
14-3-3 beta, 14-3-3 gamma, A1BG, A1M, aAmylase, ACE-2, ACLP, ACTN2, ADAM-17, ADAM-9, ADNP, ADRB2, Akt1, ALDH2, ALOX5, alpha 2u-Globulin, Alpha-fetoprotein, ALPP, AMBP, AMH, Amphiphysin, AMPK beta 1, Angiogenin, Angiopoietin-2, Angiotensinogen, Annexin A11, Annexin A2, ApoH, ARC, ATF2, ATF6, ATG3, ATM, Axin-1, B7-H4, BAG4, BAG6, BAMBI, BarX1, BCHE, Beclin 1, beta-Actin, beta-I Tubulin, BMX, BNIP3L, BOK, Brevican, Cadherin-15, Cadherin-8, CALD1, Calretinin, CaM Kinase II, CaMKK alpha, CapG, Carbonic Anhydrase XIV, Carboxypeptidase M, CART, Cathepsin G, Caveolin-1, CBP, CCBL1, CCR2, CCR6, CCR8, CCR9, CD106, CD161, CD164, CD19, CD28, CD29, CD36, CD39L4, CD40, CD44, CD51, CD59, CDC25A, CDC25C, CDK1, CDK2, CEACAM-1, CELF1, CES3, CHORDC1, CKBB, CLEC1B, CLEC5A, COL1A1, COL6A1, COLEC10, Complexin-1, Contactin-3, COPZ1, CPEB3, CSNK1A, CSNK1D, CSNK1E, CSNK1G, CSNK2B, CSRP1, CXCR3, CXCR6, CYTL1, DARC, DARPP-32, DDC, DDT, DDX1, DEFA6, DGK-gamma, DGK-theta, DISC 1, DKK-1, Dkk-2, DOCK1, DOT1L, DRAK2, Draxin, DSC2, DYRK1A, Dystroglycan, EDN, EFEMP2, EGLN1, EIF3D, ELAVL1, Endoglin, Endophilin A1, Endorepellin, ENSA, EphA3, Ephrin-B3, ErbB4, ERK1, ERK2, ERK4, FAIM3, FANCD2, Fascin, FASN, FBPase 1, FCGR2B, FCGR3A, FCGRT, Fen 1, Filamin A, FKBPR38, FoxA2, FoxP3, FPRP, FSTL4, FUCA1, Fyn, G3BP, G6PD, GABAB R1, GABAB R2, GABRA1, GABRA5, GALNT2, gamma-Catenin, GATE-16, GBL, GDF1, GDF-7, GDI1, Gephyrin, GLUD1, Glycine R, GOLGB1, GPLD1, GPR64, GPX2, GPX4, GRB7, GRP78, GSK-3 alpha, H6PD, HABP2, HAO-1, HBB, HCLS1, HDAC2, HDAC4, Heparan Sulfate 6-O-Sulfotransferase 3, HHEX, HIBADH, HIF-2 alpha, Histamine H3 R, Histone H1.3, Histone H2AX, HMGB1, HMGN2, HMOX1, HN1, hnRNP G, hnRNP U, HOMER1, HP1BP3, HPRT, HSP90B1, HSPA2, HSPB8, IBP160, ICAM-5, IKK alpha, IKK-gamma, IL-12 R beta 2, IL-17 RA, IL-17F, IL-20 R beta, IL-21 R, IL-6 R, IMPA1, IMPAD1, IMPDH2, Inhibin beta, iNOS, Intelectin-1, IRE1, IRS1, IRS2, ITGA8, ITGB4BP, ITGB5, ITGB6, ITPR3, JAB1, Jak1, JIP1, Kallikrein 7, KCNB2, KCNC1, KIAA1967, Klotho beta, KMO, KOR, KPNB1, Kynureninase, LAMC1, Laminin S, LC3B, LHX5, LIPG, Lipin 2, LMAN1L, LMNA, LOK, LRPAP, Lumican, Lysozyme, LYVE-1, MAD2L1, MafB, MAP4K4, Matrilin-4, MBP, MCHR1, M-CSF, MDGA2, MDH1, MDM2, MEK1, MEKK2, MESDC2, Metallothionein, mGluR1, mGluR2/3, mGluR5, MIB1, MIOS, MIP-1 beta, MKP-3, MLK4, MN1, MPP5, M-Ras, MSH6, Musashi-1, MyD88, MYHC, Myoglobin, NAP1L1, NBS1, NCAM2, NCOR1, NDFIP1, Nectin-3, Nesfatin-1, Nesprin2, Neurogenin-2, Neuroglycan C, NGFR, Nicalin, Ninjurin-2, NIPP1, NKX2.2, NLRP10, NPC1, NR3C1, NrCAM, NSE, NT5E, NUAK1, Nucleostemin, NXPH3, Oligodendrocyte Marker O1, Oligodendrocyte Marker O4, Oncomodulin, OPRM1, Osteopontin, OV-6, P20Sb3, p38 alpha, p70 S6 Kinase, PABP, PAK4, PAK6, Pannexin-1, Park7, PARL, Parvalbumin, Paxillin, PCBP2, PCDH12, PCK2, PCNA, PDAP1, PDCD5, PDHX, PDK-1, PDX-1, PDZK1, Perilipin-1, PGAM2, PGCD6, PGK1, PGLS, PGM1, PGRPs, PIK3R1, PIWIL2, PKA RI beta, PKC beta 1, PKC gamma, PKLR, PKN2, PLA2G2A, PLC-beta 4, PLC-gamma 1, Plexin A1, Plexin A2, Plexin A3, Plexin B3, PLOD2, PLTP, PNPLA2, PNUTS, PP2A, PPM1B, PPM1L, PPP1R9B, PRCP, PRDX5, PRG2, PRNP, Prohibitin, Proprotein Convertase 9, Prss21, PSD-95, PTEN, PTGDS, PTGES3, PTP gamma, PTP-MEG2, PTPRM, PTPRU, PVRL1, QDPR, Rab11A, Rab27a, RAB7A, RAC1, RACK1, Rad17, Raf-1, Rap1A/B, Rap2A/B, RECQ4, Reg4, Relaxin R1, RELM gamma, RGM-B, RGM-C, RHOG, RIBP, RIPK1, RKIP, RNASE4, RNF2, ROCK1, RPL10A, RPL11, RPL22, RPLP2, RPS11, RPS19, RPS25, RPS4X, RPS6, RRAS2, RSK1, RSK2, RTN1-A, Ryk, SC35, SCGB3A1, SCGF, SEC13, SECISBP2, SEMA3F, SENP8, Serpin A3N, Serpin A6, Serpin D1, Serpin E2, Serpin A12, SerRS, SGSH, SHC1, SHIP, SHP-2, SIGNR3, Six3, SMAGP, SMOC-1, SMURF2, SNAP25, SOD1, SOD2, SOD3, SPOCK2, SQSTM1, SR-AI, ST3GAL2, STAT5b, STAT6, STI1, STIM1, STK3, Substance P, SUMO3, SUSD2, Synaptotagmin-1, Syndecan-3, Syntaxin 1B, Syntaxin 7, Syntaxin 8, Syntaxin BP1, T Cell Receptor alpha Chain-V, TCEB2, TCP1 eta, Tenascin R, TfR, TGN38, TH, Themis, Thioredoxin-1, Thrombopoietin, TLR7, TOP2B, TOR, TRIM63, TROP1, Troponin T, TRP14, TRPV1, TRXR1, Trypsin 3, Trypsin Pan, TSC22, TSH, TXNDC4, UBASH3B, UBE2N, UQCRB, UROD, VAP-B, VE-Cadherin, Versican, Vimentin, WNK1, WNK2, WT1, WWOX, XPB, YAP1, Yes, ZBTB4
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Rat Tyrosine Phosphorylation Antibody Array G4 Slide(s)
- 2X Cell Lysis Buffer
- Protease Inhibitor Cocktail
- Phosphatase Inhibitor Cocktail Set II
- Blocking Buffer
- 20X Wash Buffer I
- 20X Wash Buffer II
- Biotin-Conjugated Anti-phosphotyrosine
- Fluorescent dye-Conjugated Streptavidin
- Wash Buffer III
- Adhesive film
Other Materials Required
- Shaker
- Laser scanner for fluorescence detection
- Aluminum foil
- Distilled water
- Plastic box
- 50 ml Centrifuge tube
- Isopropanol (2-propanol)
Protocol Outline
- Dry the Glass Slide
- Blocking and Incubation
- Fluorescence Detection
Storage/Stability
Upon receipt, the kit should be stored at -20°C to -80°C. Please use within 6 months from the date of shipment. After initial use, the 2X Cell Lysis Buffer, Blocking Buffer, 20X Wash Buffer I, 20X Wash Buffer II, Biotin-Conjugated Anti-phosphotyrosine and Fluorescent dye-Conjugated Streptavidin (Cy3 equivalent) should be stored at 4°C to avoid repeated freeze-thaw cycles. The Array I Glass Slide, Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail Set II should be kept at -20°C to -80°C. Use within 3 months after initial use.