• 4 hour processing time
• 5-10x more cost-effective than ELISA
• Compatible with most flow cytometers
• No specialized or dedicated instrument(s) needed
• High specificity and system reproducibility
• Well-suited for high throughput assays

• Capture Beads
• Filler Beads
• Assay Diluent
• Wash Buffer
• Lyophilized Protein Standard
• Biotinylated Detection Antibody
• PE Conjugate
• Flow Cytometer Setup Bead
• V-bottom 96-well Microplate

• Orbital 96-well plate shaker (with ability to reach 1000 rpm)
• Flow cytometer with blue (or green) capable of measuring phycoerythrin (PE)
• Aluminum foil or 96-well Plate Aluminum Sealers
• Deionized (DI) water
• 1.5 mL polypropylene microcentrifuge tubes or similar
• Microcentrifuge
• Table centrifuge with plate holder
• Recommended: Vacuum manifold compatible with a 96-well microplate if using a Filter 96-Well Microplate
• Optional: 96-well round bottom plate (to prepare samples)
• Optional: High throughput sampler (HTS) for 96-well plate reading

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. Get a quote!

1. Prepare Reagents, Standards, and Samples
2. Add Capture Beads to each well
3. Incubate with Samples and Standards
4. Incubate with Biotinylated Detection Antibody
5. Incubate with PE Conjugate
6. Analyze with a compatible flow cytometer
7. Perform data analysis