• Less sample, more data: up to 27 proteins can be quantified from only 25 µL or less of your precious sample
• 4 hour processing time
• 5-10x more cost-effective than ELISA
• Compatible with most flow cytometers
• No specialized or dedicated instrument(s) needed
• High specificity and system reproducibility
• Well-suited for high throughput assays

• RayPlex^® Magnetic Multiplex Bead Cocktail
• Assay Diluent
• Wash Buffer
• RayBio^® Serum/Plasma Diluent
• Lyophilized Protein Standard Mix
• Biotinylated Detection Antibody Cocktail
• PE Conjugate
• Flow Cytometer Setup Bead Cocktail
• V-bottom 96-well Microplate
• Flat-bottom 96-well Microplate

• Orbital 96-well plate shaker (with ability to reach 1000 rpm)
• Flow Cytometer with blue (or green) and red lasers capable of measuring phycoerythrin (PE), Alexa Fluor 700 (AF700), and Alexa Fluor 647 (AF647)/APC channels OR Luminex instrument
• Aluminum foil or 96-well Plate Aluminum Sealers
• PBS
• Deionized (DI) water
• 1.5 mL polypropylene microcentrifuge tubes or similar
• Microcentrifuge
• Recommended: Automated plate washing system with magnetic separation
• Optional: 96-well round bottom plate (to prepare samples)
• Optional: High throughput sampler (HTS) for 96-well plate reading
• Optional: Vacuum manifold compatible with a 96-well microplate if using a Filter 96-Well Microplate

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. Get a quote!

1. Prepare Reagents, Standards, and Samples
2. Add RayPlex^® Magnetic Multiplex Bead Cocktail to each well
3. Incubate with Samples and Standards
4. Incubate with Biotinylated Detection Antibody Cocktail
5. Incubate with PE Conjugate
6. Analyze with a compatible flow cytometer
7. Perform data analysis

Upon receipt, the Lyophilized Protein Standard Mix should be stored at -20°C or below. Other components should be stored at 4°C. Use reagents immediately after preparation. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.