RayBiotech

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ISO 13485:2016 cGMP

Recombinant Bovine TNF ALPHA

Catalog #: 229-20107

Product Description

Specifications

Size20 µg
Estimated Lead Time1-2 weeks
SpeciesBovine (cow)
Protein Name / Synonyms
TNF ALPHA,
Expression SystemE. coli
Purity
>95% by SDS PAGE analysis
Molecular Weight (kDa)
17.4kD
Format
Purified Recombinant Protein- lyophilized
Preservative
None present
Recommended ApplicationsELISA, Functional Assays, ELISA, Functional Assays
Endotoxin Levelless than 0.04 EU/ug
Shipping TypeAmbient temperature
Storage-70°C

Description

Introduction

Bovine Tumour Necrosis Factor alpha (TNF alpha) is produced by a range of cells, including macrophages, monocytes, T and B lymphocytes. TNF alpha is an important mediator of the bovine inflammatory response.

129-20107 has an ED50 in the range of 10-25 ng/ml, determined by measuring the cytolytic effect of recombinant bovine TNF alpha on murine WEHI-13VAR cells in the presence of Actinomycin D.

Specifications

Size
20 ug
Assay Buffers
Phosphate buffered saline

Stability & Storage

Storage/Stability
Prior to reconstitution store at +4°C.
Following reconstitution store at -70°C.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for12 months from date of reconstitution.