Product Description

Specifications

Size	500 µg
Estimated Lead Time	1-2 business days
Species	Human
Accession Number	Q7L553
Gene Symbols	FN1
Protein Name / Synonyms	Fibronectin, FN1
Expressed Region	Lys1-Glu211
Expression System	E. coli
Tag	N-terminal His-tag
Purity	>90%
Purity Determined By	SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa)	45 kDa
Format	Lyophilized powder
Formulation	Lyophilized from a 0.2 µm filtered solution in 1x PBS (pH 7.5).
Concentration (lot specific)	Prior to lyophilization, the protein concentration was measured by Bio-Rad Bradford protein dye reagent concentrate.
Reconstitution	Briefly spin the vial to ensure all contents are at the bottom before opening. It is recommended to reconstitute the product at a concentration of 0.5-1.0 mg/mL using sterile deionized water.
Research Area	Cell Adhesion
Shipping Type	Blue ice
Storage	-20°C

SDS-PAGE Image

Activity

Measured in a cell adhesion assay using NIH-3T3 mouse embryonic fibroblast cells. Recombinant Fibronectin can improve cell attachment significantly.

Storage/Stability

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long-term storage, it is recommended to store the desiccated product below -20°C in a manual defrost freezer. Generally, the shelf life is up to 12 months from the date of receipt when stored at -20°C or -80°C under sterile conditions. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to prepare appropriate aliquots and store them at -20°C or -80°C.