Recombinant HBV A 2 envelope protein, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 50 µg, 100 µg, 200 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Hepatitis B virus (HBV) |
| Specificity | Hepatitis B virus (HBV) genotype A2 subtype adw |
| Accession Number | P03142 |
| Gene Symbols | S |
| Protein Name / Synonyms | HBV Large Envelope Protein (B1 subtype), L glycoprotein, L-HBsAg, Large S protein |
| Expressed Region | Met1-Ser169 |
| Expression System | HEK293 cells |
| Tag | C-terminal his-tag |
| Purity | >90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of ∼18 kDa. Due to the abundant glycosylation, it migrates as approximately ∼30-35 kDa protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under native and denature conditions, the protein presented as ∼20-25 kDa band smear. See deglycosylation analysis image below. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS (pH 7.4) |
| Concentration (lot specific) | Determined by BCA protein assay |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water. |
| Endotoxin Level | < 0.5 EU per µg of the protein as determined by the LAL method |
| Research Area | Infectious Disease, Hepatitis |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Lane 1: protein standard ladder (kDa).
Lane 2: untreated protein.
Lane 3: treated protein with deglycosylation enzymes under native conditions.
Lane 4: treated protein with deglycosylation enzymes under denature conditions.
Lane 5: deglycosylation enzymes only without target proteins.
Stability & Storage
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.
Custom Labeling Request
We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.
