Biotin-conjugated recombinant HBV (B1 subtype) envelope protein with C-terminal His-tag, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 50 µg, 100 µg, 200 µg |
| Estimated Lead Time | 2-3 business days |
| Species | Hepatitis B virus (HBV) |
| Specificity | Hepatitis B virus (HBV) B1 subtype |
| Accession Number | P17398 |
| Gene Symbols | S |
| Protein Name / Synonyms | HBV Large Envelope Protein (B1 subtype), L glycoprotein, L-HBsAg, Large S protein |
| Expressed Region | Met1-Ser169 |
| Expression System | HEK293 cells |
| Conjugation | Biotin |
| Tag | C-terminal his-tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method. |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of ∼20 kDa. Due to the abundant glycosylation, it migrates as approximately 20-40 kDa protein smear bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under native and denature conditions, the protein presented as one reduced ∼20 kDa band. See deglycosylation analysis image below. |
| Format | Lyophilized powder (slight pink) |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS (pH 7.4) |
| Concentration (lot specific) | Determined by BCA protein assay |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water. |
| Endotoxin Level | < 0.5 EU per µg of the protein as determined by the LAL method |
| Research Area | Infectious Disease, Hepatitis |
| Protein Type | Recombinant Protein |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Stability & Storage
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.
