Biotin-conjugated recombinant Human HIF-1-alpha, derived from the transfected human HEK293 cells
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Product Description
Specifications
| Size | 10 µg, 50 µg, 100 µg |
| Estimated Lead Time | 2-3 business days |
| Species | Human |
| Accession Number | Q16665 |
| Gene Id | 3091 |
| Gene Symbols | HIF1A |
| Protein Name / Synonyms | Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, Member of PAS protein 1, PAS domain-containing protein 8, Hypoxia Inducible Factor 1, Alpha Subunit, Basic Helix-Loop-Helix Transcription Factor, HIF-1alpha, MOP1, HIF1, ARNT Interacting Protein, Hypoxia-Inducible Factor 1 Alpha Isoform I.3, PASD8, Hypoxia-Inducible Factor1alpha, Member Of PAS Superfamily 1, PAS Domain-Containing Protein 8, BHLHE78, HIF-1A |
| Expressed Region | Leu400-Asn826 |
| Expression System | HEK293 cells |
| Conjugation | Biotin |
| Tag | N-terminal 8x histidine tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method. |
| Purity | >90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | The recombinant human HIF-1-alpha, C-terminal domain, has a calculated molecular mass of 49 kDa. Due to the abundant post-translation modifications, it migrates as approximately 60-80 kDa protein bands (confirmed by Western blotting) in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS |
| Concentration (lot specific) | Determined by BCA protein assay kit (Thermo Scientific) |
| Preservative | None |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS. |
| Endotoxin Level | Not determined |
| Research Area | Angiogenesis, Cardiovascular Disease, Inflammation, HIF-1 alpha Signaling, Autophagy, Hypoxia Biology |
| Protein Type | Recombinant Protein |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Stability & Storage
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.
