Recombinant human 14-3-3 protein theta with a C-terminal His-tag, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 10 µg, 100 µg, 250 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P27348 |
| Gene Symbols | YWHAQ |
| Protein Name / Synonyms | 14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1. |
| Expressed Region | Glu2- Asn245 |
| Expression System | HEK293 cells |
| Tag | C-terminal His-tag |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 30 kDa. Due to the abundant glycosylation, it migrates as approximately 37 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Format | Liquid |
| Formulation | Supplied as a 0.2 µm filtered solution in 1x PBS (pH 7.2) with 1% mannitol and 5% trehalose |
| Concentration (lot specific) | Refer to the label on the vial for the specific concentration, determined by the Pierce BCA Protein Assay Kit. |
| Endotoxin Level | < 0.5 EU per μg of the protein as determined by the LAL method |
| Shipping Type | Blue ice |
| Storage | -20°C |
SDS-PAGE Image
Storage/Stability
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Upon arrival, the liquid protein may be stored for 2 weeks at 4°C. For long-term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Generally, the shelf life is up to 6 months from the date of receipt at -20°C or -80°C under sterile condition.