Biotin-conjugated recombinant human Alpha-2-MRAP protein with a C-terminal His-tag, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 200 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P30533 |
| Gene Id | 4043 |
| Gene Symbols | LRPAP1 A2MRAP |
| Protein Name / Synonyms | Alpha-2-macroglobulin receptor-associated protein (Alpha-2-MRAP) (Low density lipoprotein receptor-related protein-associated protein 1) (RAP) |
| Expressed Region | Tyr35-Leu357 |
| Expression System | HEK293 cells |
| Conjugation | Biotin |
| Tag | C-terminal His-tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method. |
| Purity | >90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 38 kDa. For some unknown reasons, the purified proteins presented with 2 major bands in SDS-PAGE. Due to the abundant glycosylation, it migrates as approximately 30 kDa and 40 kDa bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Format | Liquid |
| Formulation | Filtered 0.2 µm solution in 1x PBS (pH 7.2) with 1% mannitol and 5% trehalose, 20% glycerol |
| Concentration (lot specific) | Lot specific. See vial label, determined by Bio-Rad Bradford protein dye reagent concentrate. |
| Endotoxin Level | < 0.5 EU per μg of the protein as determined by the LAL method |
| Research Area | Ras/Rap Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
SDS-PAGE Image
Storage/Stability
Upon arrival, the liquid protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.