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Amyloid beta A4 protein (ABPP) (APPI) (APP) (Alzheimer disease amyloid protein) (Amyloid precursor protein) (Beta-amyloid precursor protein) (Cerebral vascular amyloid peptide) (CVAP) (PreA4) (Protease nexin-II) (PN-II) [Cleaved into: N-APP, Soluble APP-a, Amyloid beta A4 protein, ABPP, APPI, APP, Alzheimer disease amyloid protein, Amyloid precursor proteinCurated, Beta-amyloid precursor protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, Amyloid Beta Precursor Protein, Amyloid Beta (A4) Precursor Protein, Amyloid Precursor Protein, Peptidase Nexin-II, CVAP, AD1, Testicular Tissue Protein Li 2, Beta-Amyloid Peptide(1-40), Beta-Amyloid Peptide(1-42), Beta-Amyloid Peptide, Alzheimer Disease, CTFgamma, ABETA, AAA, PN2, A4

Unpurified cell culture supernatant. HEK293 cells grown in serum-free medium were transfected with expression vector harboring target gene. The cell culture was harvested with centrifugation to remove cells. The cell culture supernatant containing mammalian cell protease inhibitor cocktail was aliquoted and stored at -80°C immediately. The gene overexpression in culture supernatant was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the culture supernatant derived from HEK293 cells transfected with the empty expression vector was used as a negative control.

Recombinant protein has a calculated molecular weight of about 77 kDa. The actual molecular weight may increase slightly due to the potential post-modifications (PTMs).

Culture supernatant of transfected HEK293 cells

None