328

DNA-(apurinic or apyrimidinic site) lyase (EC 3.1.-.-) (EC 4.2.99.18) (APEX nuclease) (APEN) (Apurinic-apyrimidinic endonuclease 1) (AP endonuclease 1) (APE-1) (REF-1) (Redox factor-1) [Cleaved into: DNA-(apurinic or apyrimidinic site) lyase, mitochondria, DNA-(apurinic or apyrimidinic site) lyase, APEX nuclease, APEN, Apurinic-apyrimidinic endonuclease 1, AP endonuclease 1, APE-1, REF-1, Redox factor-1, Apurinic/Apyrimidinic Endodeoxyribonuclease 1, APEX Nuclease (Multifunctional DNA Repair Enzyme) 1, APEX, APE1, HAP1, REF1, APE, APX, APEX Nuclease (Multifunctional DNA Repair Enzyme), Deoxyribonuclease (Apurinic Or Apyrimidinic), Apurinic/Apyrimidinic (Abasic) Endonuclease, AP Endonuclease Class I, Protein REF-1, AP Lyase

Unpurified cell culture supernatant. HEK293 cells grown in serum-free medium were transfected with expression vector harboring target gene. The cell culture was harvested with centrifugation to remove cells. The cell culture supernatant containing mammalian cell protease inhibitor cocktail was aliquoted and stored at -80°C immediately. The gene overexpression in culture supernatant was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the culture supernatant derived from HEK293 cells transfected with the empty expression vector was used as a negative control.

Recombinant protein has a calculated molecular weight of about 36 kDa. The actual molecular weight may increase slightly due to the potential post-modifications (PTMs).

Culture supernatant of transfected HEK293 cells

None