Recombinant human CD86 protein with a C-terminal His-tag, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P42081 |
| Gene Id | 942 |
| Gene Symbols | CD86 CD28LG2 |
| Protein Name / Synonyms | T-lymphocyte activation antigen CD86 (Activation B7-2 antigen) (B70) (BU63) (CTLA-4 counter-receptor B7.2) (FUN-1) (CD antigen CD86) |
| Expressed Region | Ala24-Pro247 |
| Expression System | HEK293 cells |
| Tag | C-terminal His-tag |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 28 kDa. Due to the abundant glycosylation, it migrates as approximately 50-55 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Format | Liquid |
| Formulation | Filtered 0.2 µm solution in 1x PBS (pH 7.2) with 1% mannitol and 5% trehalose |
| Concentration (lot specific) | Lot specific. See vial label, determined by BCA protein assay reagent. |
| Endotoxin Level | < 0.5 EU per μg of the protein as determined by the LAL method |
| Shipping Type | Blue ice |
| Storage | -20°C |
SDS-PAGE Image
Storage/Stability
Upon arrival, the liquid protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.