Recombinant human cerberus 1, cysteine knot superfamily, homolog (Xenopus laevis), derived from transfected human HEK293 cells.
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Product Description
Specifications
| Size | 10 µg, 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | O95813 |
| Gene Symbols | CER1 |
| Protein Name / Synonyms | Cerberus, Cerberus 1, DAN Family BMP Antagonist, DAN Domain Family Member 4, Cerberus-Related Protein, DAND4, Cerberus 1 (Xenopus Laevis) Homolog (Cysteine Knot Superfamily), Cerberus 1, Cysteine Knot Superfamily, Homolog (Xenopus Laevis), Cerberus 1, Cysteine Knot Superfamily, Homolog, Cerberus-Related 1 |
| Expressed Region | Thr18-Ala267 |
| Expression System | HEK293 cells |
| Tag | N-terminal histidine tag |
| Purity | >90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant human cerberus protein has a calculated molecular mass of 24 kDa. Due to the abundant glycosylation, it migrates as approximately 37-45 kDa protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS |
| Concentration (lot specific) | Determined by Pierce BCA protein assay kit |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Lane 1: Protein standard ladder (kDa)
Lane 2: Untreated protein under reducing conditions
Lane 3: Treated protein with deglycosylation enzymes under native conditions
Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.
Lane 5: Deglycosylation mixture only without target proteins.
Stability & Storage
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.
Custom Labeling Request
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