Recombinant Human Ceruloplasmin Protein

Recombinant human ceruloplasmin protein, derived from the transfected human HEK293 cells.
As low as $127.00
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Product Description


Size 10 ug, 50 ug, 100 ug
Species Human
Tag N-terminal His-tag
Format Lyophilized powder
Formulation Lyophilized from a 0.2 µm filtered solution in PBS (pH 7.4)
Protein Name / Synonyms Human Ceruloplasmin Protein, Ceruloplasmin, Ceruloplasmin (Ferroxidase), Ferroxidase, EC, CP-2.
Accession Number P00450
Gene Symbols CP
Concentration (lot specific) Determined by BCA protein assay
Purity >95%
Purity Determined By SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Reconstitution Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.
Expressed Region Lys20-Gly1065
Expression System Human embryonic kidney 293 (HEK293) cells
Molecular Weight Recombinant protein product has a calculated molecular mass of ∼120 kDa. Due to the abundant glycosylation, it migrates as approximately ∼140 kDa protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under native and denature conditions, the protein presented as one reduced ∼120 kDa band. See deglycosylation analysis image below.
Purification His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Figure 1. Deglycosylation analysis of purified recombinant proteins. The same amount of purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one reduced band at the expected size (ˆ¼120 kDa), thus indicating that the untreated recombinant protein (Lane 2, ˆ¼140 kDa) was glycosylated.
Lane 1, protein standard ladder (kDa).
Lane 2, untreated protein.
Lane 3, treated protein with deglycosylation enzymes under native conditions.
Lane 4, treated protein with deglycosylation enzymes under denature conditions.
Lane 5, deglycosylation enzymes only without target proteins.

Stability & Storage

Ice packs
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.

Custom Labeling Request

We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.
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