Recombinant Human Ceruloplasmin Protein
Recombinant human ceruloplasmin protein, derived from the transfected human HEK293 cells.
Product Description
Specifications
| Size | 10 ug, 50 ug, 100 ug |
|---|---|
| Species | Human |
| Tag | N-terminal His-tag |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS (pH 7.4) |
| Protein Name / Synonyms | Human Ceruloplasmin Protein, Ceruloplasmin, Ceruloplasmin (Ferroxidase), Ferroxidase, EC 1.16.3.1, CP-2. |
| Accession Number | P00450 |
| Gene Symbols | CP |
| Concentration (lot specific) | Determined by BCA protein assay |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water. |
| Expressed Region | Lys20-Gly1065 |
| Expression System | Human embryonic kidney 293 (HEK293) cells |
| Molecular Weight | Recombinant protein product has a calculated molecular mass of ∼120 kDa. Due to the abundant glycosylation, it migrates as approximately ∼140 kDa protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under native and denature conditions, the protein presented as one reduced ∼120 kDa band. See deglycosylation analysis image below. |
| Purification | His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC) |
