Biotin-conjugated recombinant Human Choriogonadotropin Subunit Beta 3 (CG-Beta), HEK293 with N-terminal His-tag, derived from HEK293 cells.
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Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P0DN86 |
| Gene Id | 1082, 93659, 94115 |
| Gene Symbols | CGB3 CGB; CGB5; CGB8 |
| Protein Name / Synonyms | Choriogonadotropin subunit beta 3 (Choriogonadotropin subunit beta) (CG-beta) (Chorionic gonadotropin chain beta) |
| Expressed Region | Ser21-Gln165 |
| Expression System | HEK293 cells |
| Conjugation | Biotin |
| Tag | N-terminal His-tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method. |
| Purity | >90 % |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining. See gel image below. |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 18 kDa. Due to the abundant glycosylation, it migrates as approximately 30-35 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below. |
| Format | Liquid |
| Formulation | Filtered solution in 1x PBS (pH 7.4) with 1% mannitol and 5% trehalose |
| Concentration (lot specific) | Lot specific. See vial labels. |
| Endotoxin Level | < 0.5 EU per μg of the protein as determined by the LAL method |
| Shipping Type | Blue ice |
| Storage | -20°C |
SDS-PAGE Image
Protein Concentration Determined By
Determined by BCA protein assay reagent
Storage/Stability
Upon arrival, the liquid protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.