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Recombinant Human CKM

Recombinant Human Creatine Kinase (Muscle)

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230-00038-10
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Source

Gene Symbols:
  • CKM
Species:
Human
Accession Number:
  • P06732
Gene ID:
  • 1158
Expressed Region:
Pro2-Asn381 (full length)
Protein Name & Synonyms:
Creatine kinase, muscle, creatine kinase-M, creatine kinase M chain, CKMM, creatine kinase M-type.

Preparation

Expression System:
E.Coli
Conjugation/Tag:
N-terminal 6x histidine tag
Purification:
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity:
> 95%
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa):
45

Protein Specifications

Format:
Lyophilized powder
Formulation:
Lyophilized from a 0.2 um filtered solution of 40 mM Tris-HCl (pH 8.2) and 50 mM NaCl with a protein concentration of 1.1 mg/mL
Reconstitution:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.1~ 1 mg/mL with sterile ice-cold deionized water or 1X Phosphate Buffered Saline (PBS). This solution can then be further diluted into other aqueous buffers.
Endotoxin Level:
Not determined
Preservative:
None

Stability & Storage

Shipping
Ice packs
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.
  1. Mitsakakis, Konstantinos, and Electra Gizeli. "Detection of multiple cardiac markers with an integrated acoustic platform for cardiovascular risk assessment." Analytica chimica acta 699.1 (2011): 1-5.
    Species: Human
    Sample type:
  2. Zhang, Guo-Jun, et al. "An integrated chip for rapid, sensitive, and multiplexed detection of cardiac biomarkers from fingerprick blood." Biosensors and Bioelectronics 28.1 (2011): 459-463.
    Species: Human
    Sample type:

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.