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Recombinant Human CNTF
Catalog #: 229-20182
Purified recombinant CTNF expressed in E. Coli
Product Description
Specifications
| Size | 20 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Protein Name / Synonyms | CNTF, |
| Expression System | E. coli |
| Purity | >98% by SDS PAGE/HPLC analysis |
| Molecular Weight (kDa) | 22.7kD |
| Format | Purified recombinant protein - lyophilized |
| Concentration (lot specific) | Total protein concentration ~0.1mg/ml after reconstitution |
| Preservative | None present. |
| Activity | The ED50 as determined by the dose-dependent proliferation of human TF-1 cells is 50-150ng/ml. |
| Recommended Applications | ELISA (0.2-0.4ng/well), Functional Assays (50-150ng/ml), Western Blotting (1.5-3.0ng/lane), ELISA (0.2-0.4ng/well), Functional Assays (50-150ng/ml), Western Blotting (1.5-3.0ng/lane) |
| Endotoxin Level | <1EU/ug |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
This product is a 199 amino acid, Escherichia coli-derived recombinant protein corresponding to mature human ciliary neutotrophic factor (CTNF), a survival factor for various neuronal cell types believed to prevent the degeneration of motor axons after axotomy.
Specifications
Size
20 ug
Assay Buffers
Phosphate buffered saline
Stability & Storage
Storage/Stability
Prior to reconstitution store at +4°C.
After reconstitution store at -20°C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution.
After reconstitution store at -20°C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution.