P00734

Coagulation Factor II, Thrombin, Prepro-Coagulation Factor II, Coagulation Factor II, EC 3.4.21.5, Coagulation Factor II (Thrombin), Prothrombin B-Chain, Serine Protease, Prothrombin, EC 3.4.21, RPRGL2, THPH1, PT.

Unpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20,000 g centrifugation at 4°C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80°C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control.

Lyophilized powder

Determined by BCA protein assay