Biotin-conjugated recombinant human coagulation factor III with an N-terminal His-tag, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 100 µg, 250 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P13726 |
| Gene Symbols | F3 |
| Protein Name / Synonyms | CD142, Coagulation factor III (thromboplastin, tissue factor) |
| Expressed Region | Ser33-Glu251 |
| Expression System | HEK293 cells |
| Conjugation | Biotin |
| Tag | N-terminal histidine tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method. |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 25 kDa. Due to the abundant glycosylation, it migrates as approximately 20-25 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below. |
| Format | Liquid |
| Formulation | Supplied as a 0.2 µm filtered solution in PBS (pH 7.4) with 1% mannitol and 5% trehalose |
| Concentration (lot specific) | Lot specific (see the label on the vial), determined by Bradford protein assay |
| Endotoxin Level | <0.5 EU per µg of the protein as determined by the LAL method |
| Research Area | Angiogenesis, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
SDS-PAGE Image
Custom Labeling Request
We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.