Recombinant human collagen alpha-1(XXV) chain protein with a C-terminal His-tag, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 10 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | Q8NE08 |
| Gene Symbols | COL25A1 |
| Protein Name / Synonyms | Alzheimer disease amyloid-associated protein (AMY), CLAC-P, CLAC. |
| Expressed Region | Lys55-Arg496 |
| Expression System | HEK293 cells |
| Tag | C-terminal His-tag |
| Purity | >90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 50 kDa. Due to the abundant glycosylation, it migrates as approximately 55 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Format | Liquid |
| Formulation | Supplied as a 0.2 µm filtered solution in 1x PBS (pH 7.4) with 1% mannitol and 5% trehalose |
| Concentration (lot specific) | Refer to the label on the vial for the specific concentration, determined by Bio-Rad Bradford protein dye reagent concentrate. |
| Endotoxin Level | < 0.5 EU per μg of the protein as determined by the LAL method |
| Shipping Type | Blue ice |
| Storage | -20°C |
SDS-PAGE Image
Storage/Stability
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Upon arrival, the liquid protein may be stored for 2 weeks at 4°C. For long-term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Generally, the shelf life is up to 6 months from the date of receipt at -20°C or -80°C under sterile condition.