Recombinant Human E-FABP Protein, Biotinylated

Biotin-conjugated recombinant human fatty acid-binding protein 5/E-FABP with a C-terminal His-tag, derived from the transfected human HEK293 cells.

Product Description

Specifications

Size	100 µg, 250 µg
Estimated Lead Time	1-2 business days
Species	Human
Accession Number	Q01469
Gene Symbols	FABP5
Protein Name / Synonyms	PA-FABP, E-FABP, PAFABP, EFABP, KFABP.
Expressed Region	Ala2-Glu135
Expression System	HEK293 cells
Conjugation	Biotin
Tag	C-terminal His-tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method.
Purity	>90%
Purity Determined By	SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa)	Recombinant protein product has a calculated molecular mass of 15 kDa. Due to the abundant glycosylation, it migrates as approximately 20-30 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Format	Liquid
Formulation	Filtered solution in PBS (pH 7.4), 50 mM NaCl, 200 mM Arginine, 10% glycerol, 1% mannitol, 5% trehalose.
Concentration (lot specific)	Refer to the label on the vial for the specific concentration, determined by the Pierce BCA Protein Assay Kit.
Endotoxin Level	< 0.5 EU per μg of the protein as determined by the LAL method
Research Area	Glycolysis & Lipid Metabolism
Shipping Type	Blue ice
Storage	-20°C

SDS-PAGE Image

Figure 1. Deglycosylation analysis of purified recombinant proteins. Product was either untreated (Lane 2) or treated with deglycosylation enzymes under native (Lane 3) or denaturing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift, indicating that the untreated protein (Lane 2) was glycosylated. Lane 1, protein ladder (kDa).

Activity

Storage/Stability

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Upon arrival, the liquid protein may be stored for 2 weeks at 4°C. For long-term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Generally, the shelf life is up to 6 months from the date of receipt at -20°C or -80°C under sterile condition.