Biotin-conjugated recombinant Human Erb/Her2 (Receptor tyrosine-protein kinase erbB-2), N-terminal extracellular domain, derived from the transfected human HEK293 cells
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Product Description
Specifications
| Size | 10 µg, 50 µg, 100 µg |
| Estimated Lead Time | 2-3 business days |
| Species | Human |
| Accession Number | P04626 |
| Gene Id | 2064 |
| Gene Symbols | ERBB2 |
| Protein Name / Synonyms | V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 2, CD340, NGL, HER-2, HER2, HER-2/neu, NEU, TKR1, Neuro/Glioblastoma Derived Oncogene Homolog, C-Erb B2/Neu Protein, Metastatic Lymph Node Gene 19 Protein, herstatin, Neuroblastoma/Glioblastoma Derived Oncogene Homolog, Proto-Oncogene Neu, Receptor Tyrosine-Protein Kinase -2, Tyrosine Kinase-Type Cell Surface Receptor HER2, V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 2,Neuro/Glioblastoma Derived Oncogene Homolog, MLN 19, MLN19, p185erbB2, CD340 Antigen, V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 2, Receptor tyrosine-protein kinase erbB-2, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2 |
| Expressed Region | Thr23-Thr652 |
| Expression System | HEK293 cells |
| Conjugation | Biotin |
| Tag | N-terminal 8x histidine tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method. |
| Purity | >90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | The recombinant human ERBB2/Her2 extracellular domain has a calculated molecular mass of 71 kDa. Due to the abundant glycosylation, it migrates as an approximately 100 kDa protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS |
| Concentration (lot specific) | Determined by BCA protein assay kit (Thermo Scientific) |
| Preservative | None |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS. |
| Endotoxin Level | Not determined |
| Research Area | Angiogenesis, Cardiovascular Disease, Cancer, Phosphorylation, HER/ErbB Signaling, Tyrosine Kinase Signaling |
| Protein Type | Recombinant Protein |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Activity
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.
