2066

Erb-B2 Receptor Tyrosine Kinase 4, HER4, V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 4, Tyrosine Kinase-Type Cell Surface Receptor HER4, Human Epidermal Growth Factor Receptor 4, Proto-Oncogene-Like Protein C-ErbB-4, P180erbB4, ALS19, V-Erb-A Avian Erythroblastic Leukemia Viral Oncogene Homolog-Like 4, Avian Erythroblastic Leukemia Viral (V-Erb-B2) Oncogene Homolog 4, V-Erb-A Erythroblastic Leukemia Viral Oncogene Homolog 4, Receptor Tyrosine-Protein Kinase ErbB-4

Unpurified cell culture supernatant. HEK293 cells grown in serum-free medium were transfected with expression vector harboring target gene. The cell culture was harvested with centrifugation to remove cells. The cell culture supernatant containing mammalian cell protease inhibitor cocktail was aliquoted and stored at -80°C immediately. The gene overexpression in culture supernatant was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the culture supernatant derived from HEK293 cells transfected with the empty expression vector was used as a negative control.

Pink liquid

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