Recombinant Human GRP-78
Recombinant human 78 kDa glucose-regulated protein, derived from transfected human HEK293 cells.
Product Description
Specifications
| Size | 10 ug, 50 ug, 200 ug |
|---|---|
| Species | Human |
| Tag | N-terminal histidine tag |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS |
| Protein Name / Synonyms | GRP-78, Heat Shock 70 kDa Protein 5 (Glucose-Regulated Protein, 78kDa), GRP78, Endoplasmic Reticulum Lumenal Ca(2+)-Binding Protein Grp78, Immunoglobulin Heavy Chain-Binding Protein, BIP, Heat Shock 70kD Protein 5 (Glucose-Regulated Protein, 78kD), Epididymis Secretory Sperm Binding Protein Li 89n, 78 KDa Glucose-Regulated Protein, Heat Shock 70 KDa Protein 5, HEL-S-89n, GRP-78, MIF2 |
| Accession Number | P11021 |
| Gene Symbols | HSPA5 |
| Recommended Applications | Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc. |
| Concentration (lot specific) | Determined by Pierce BCA protein assay kit |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water. |
| Expressed Region | Glu19-Leu654 |
| Expression System | Human Embryonic Kidney 293 Cells |
| Molecular Weight | Recombinant human HSPA5 protein has a calculated molecular mass of 70 kDa. Due to the abundant glycosylation, it migrates as approximately 80-100 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Purification | His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC) |
