Recombinant Human GRP-78

Recombinant human 78 kDa glucose-regulated protein, derived from transfected human HEK293 cells.
As low as $131.00
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Product Description


Size 10 ug, 50 ug, 200 ug
Species Human
Tag N-terminal histidine tag
Format Lyophilized powder
Formulation Lyophilized from a 0.2 µm filtered solution in PBS
Protein Name / Synonyms GRP-78, Heat Shock 70 kDa Protein 5 (Glucose-Regulated Protein, 78kDa), GRP78, Endoplasmic Reticulum Lumenal Ca(2+)-Binding Protein Grp78, Immunoglobulin Heavy Chain-Binding Protein, BIP, Heat Shock 70kD Protein 5 (Glucose-Regulated Protein, 78kD), Epididymis Secretory Sperm Binding Protein Li 89n, 78 KDa Glucose-Regulated Protein, Heat Shock 70 KDa Protein 5, HEL-S-89n, GRP-78, MIF2
Accession Number P11021
Gene Symbols HSPA5
Concentration (lot specific) Determined by Pierce BCA protein assay kit
Purity >95%
Purity Determined By SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Reconstitution Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.
Expressed Region Glu19-Leu654
Expression System Human Embryonic Kidney 293 Cells
Molecular Weight Recombinant human HSPA5 protein has a calculated molecular mass of 70 kDa. Due to the abundant glycosylation, it migrates as approximately 80-100 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Purification His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with Protein Deglycosylation Kit under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.
Lane 1: Protein standard ladder (kDa)
Lane 2: Untreated protein under reducing conditions
Lane 3: Treated protein with deglycosylation enzymes under native conditions
Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.
Lane 5: Deglycosylation mixture only without target proteins.

Stability & Storage

Ice packs
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.

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