Recombinant human heat shock 60 kDa protein (HSP-60), derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 10 µg, 50 µg, 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P10809 |
| Gene Symbols | HSPD1 |
| Protein Name / Synonyms | Heat Shock 60kDa Protein 1 (Chaperonin), HSP60, Mitochondrial Matrix Protein P1, P60 Lymphocyte Protein, 60 KDa Chaperonin, Chaperonin 60, HuCHA60, HSP-60, 60 KDa Heat Shock Protein |
| Expressed Region | Ala27-Phe573 |
| Expression System | HEK293 cells |
| Tag | N-terminal his-tag |
| Purity | >90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 58 kDa. Due to the potential post-translational modifications, it migrates as approximately 75 kDa major protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS (pH 7.4) |
| Concentration (lot specific) | Determined by BCA protein assay |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water. |
| Research Area | Apoptosis, Cancer |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Introduction
Heat Shock Protein 60 (HSP60), also known as chaperonin 60, is a molecular chaperone predominantly localized in the mitochondria. It plays a crucial role in maintaining mitochondrial protein homeostasis by assisting in the proper folding and assembly of nascent polypeptides. Beyond its chaperoning functions, HSP60 is involved in various cellular processes, including thermotolerance, apoptosis, and immune responses. Notably, HSP60 has been implicated in the pathogenesis of cardiovascular diseases, cancer, and neurodegenerative disorders, highlighting its significance in both health and disease contexts.
Reference: https://link.springer.com/article/10.1007/s11033-021-06676-4
SDS-PAGE Image
Lane 1: Protein standard ladder (kDa)
Lane 2: Untreated protein under reducing conditions
Lane 3: Treated protein with deglycosylation enzymes under native conditions
Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.
Lane 5: Deglycosylation mixture only without target proteins.
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.
Custom Labeling Request
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