Recombinant human HRAS protein derived from E.coli
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Product Description
Specifications
| Size | 10 µg, 50 µg, 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P01112-1 |
| Gene Symbols | HRAS |
| Protein Name / Synonyms | Harvey Rat Sarcoma Viral Oncogene Homolog, V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog, Transforming Protein P21, C-H-RAS, P21ras, HRAS1, Ras Family Small GTP Binding Protein H-Ras, Harvey Rat Sarcoma Viral Oncoprotein, Transformation Gene: Oncogene HAMSV, GTP- And GDP-Binding Peptide B, C-Ras-Ki-2 Activated Oncogene, Ha-Ras1 Proto-Oncoprotein, C-Has/Bas P21 Protein, P19 H-RasIDX Protein, GTPase HRas, C-BAS/HAS, C-HA-RAS1, H-RASIDX, H-Ras-1, Ha-Ras, HAMSV, RASH1, CTLO |
| Expressed Region | Thr2-Ser189 |
| Expression System | E. coli |
| Tag | N-terminal 6x histidine tag |
| Purity | > 95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | 24 kDa |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.45 µm filtered solution in PBS (pH 7.4). |
| Concentration (lot specific) | Determined by BCA protein assay kit (Thermo Scientific) |
| Preservative | None |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS. |
| Endotoxin Level | Not determined |
| Research Area | Cardiovascular Disease, Cancer |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Stability & Storage
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.