Recombinant Human HSP 90-alpha, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 10 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P07900 |
| Gene Symbols | HSP90AA1 |
| Protein Name / Synonyms | HSP 90-alpha, Heat shock protein HSP 90-alpha, Heat shock 86 kDa, HSP 86, HSP86, Lipopolysaccharide-associated protein 2, LAP-2, LPS-associated protein 2, Renal carcinoma antigen NY-REN-38, Heat Shock Protein 90kDa Alpha (Cytosolic), Class A Member 1, HSPC1, HSPCA, HSP90A, Heat Shock 90kD Protein 1, Alpha, Heat Shock 90kDa Protein 1, Alpha, Heat Shock 86 KDa, Renal Carcinoma Antigen NY-REN-38, HSP89A, HSPCAL, LAP2, EL52, HSP90N, HSPCAL1, HSPN, Hsp89, Hsp90 Epididymis Luminal Secretory Protein 52, Heat Shock 90kD Protein 1, Alpha-Like 4, Heat Shock 90kD Protein, Alpha-Like 4, Heat Shock Protein HSP 90-Alpha |
| Expressed Region | Pro2-Asp732 |
| Expression System | HEK293 cells |
| Tag | N-terminal histidine tag |
| Purity | >90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant Human HSP 90-alpha has a calculated molecular mass of 87 kDa. Due to the abundant glycosylation, it migrates as approximately 100-150 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS |
| Concentration (lot specific) | Determined by Pierce BCA protein assay kit |
| Preservative | None |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water. |
| Research Area | Autophagy |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Lane 1: Protein standard ladder (kDa).
Lane 2: Untreated protein under reducing conditions.
Lane 3: Treated protein with deglycosylation enzymes under native conditions.
Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.
Lane 5: Deglycosylation mixture only without target proteins.
Stability & Storage
Shipping
Including one vial of cell culture supernatant of HEK293 cells transfected with empty expression vector.
Reconstitution
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.
Custom Labeling Request
We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.