3326

Heat shock protein HSP 90-beta (HSP 90) (Heat shock 84 kDa) (HSP 84) (HSP84), Heat shock protein HSP 90-beta, HSP 90, Heat shock 84 kDa, HSP 84, HSP84, Heat Shock Protein 90 Alpha Family Class B Member 1, Heat Shock Protein 90kDa Alpha (Cytosolic), Class B Member 1, Heat Shock Protein 90kDa Alpha Family Class B Member 1, Heat Shock 90kD Protein 1 Beta, HSP90B, HSPC2, HSPCB, Heat Shock 90kDa Protein 1 Beta, Heat Shock Protein 90 KDa, HSP90-Beta, D6S182

Unpurified cell culture supernatant. HEK293 cells grown in serum-free medium were transfected with expression vector harboring target gene. The cell culture was harvested with centrifugation to remove cells. The cell culture supernatant containing mammalian cell protease inhibitor cocktail was aliquoted and stored at -80°C immediately. The gene overexpression in culture supernatant was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the culture supernatant derived from HEK293 cells transfected with the empty expression vector was used as a negative control.

Recombinant protein has a calculated molecular weight of about 83 kDa. The actual molecular weight may increase slightly due to the potential post-modifications (PTMs).

Culture supernatant of transfected HEK293 cells

None