Biotin-conjugated recombinant human Heat shock 70 kDa protein 1A (HSPA1A) with an N-terminal His-tag, derived from the transfected human HEK293 cells.
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Figure 1. Deglycosylation analysis of purified recombinant proteins. Lane 1, protein ladder (kDa). Lane 2, glycosylated protein (delivered to customers). Lanes 3 and 4, deglycosylated proteins under native and denature conditions respectively.
| Size | 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P0DMV8 |
| Gene Symbols | HSPA1A |
| Protein Name / Synonyms | Heat shock 70 kDa protein 1A |
| Expressed Region | Ala2-Asp641 |
| Expression System | HEK293 cells |
| Conjugation | Biotin |
| Tag | N-terminal histidine tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method. |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 70 kDa. The purified product showed one reduced 55 kDa protein band in SDS-PAGE with abundant glycosylation. See deglycosylation analysis image below. |
| Format | Liquid |
| Formulation | Supplied as a 0.2 µm filtered solution in PBS (pH 7.4) with 1% mannitol and 5% trehalose |
| Concentration (lot specific) | Lot specific (see the label on the vial), determined by BCA protein assay |
| Endotoxin Level | <0.5 EU per µg of the protein as determined by the LAL method |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |

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Figure 1. Deglycosylation analysis of purified recombinant proteins. Lane 1, protein ladder (kDa). Lane 2, glycosylated protein (delivered to customers). Lanes 3 and 4, deglycosylated proteins under native and denature conditions respectively.