Biotin-conjugated recombinant human IGF-I (Insulin-like growth factor 1, somatomedin C), derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 10 µg, 50 µg, 100 µg |
| Estimated Lead Time | 2-3 business days |
| Species | Human |
| Accession Number | P05019 |
| Gene Id | 3479 |
| Gene Symbols | IGF1 |
| Protein Name / Synonyms | Insulin-like growth factor I, IGF-I, Mechano growth factor, MGF, Somatomedin-C, Insulin-Like Growth Factor 1, IBP1, IGFI |
| Expressed Region | Gly49-Lys195 |
| Expression System | HEK293 cells |
| Conjugation | Biotin |
| Tag | N-terminal 6x histidine tag and C-terminal mouse IgG Fc tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method. |
| Purity | >90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant mouse IGF-I has a calculated molecular mass of 43 kDa (including a C-terminal mouse IgG Fc tag, 27 kDa). Due to the abundant glycosylation, it migrates as approximately 43-45 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS |
| Concentration (lot specific) | Determined by BCA protein assay kit (Thermo Scientific) |
| Preservative | None |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS. |
| Endotoxin Level | Not determined |
| Research Area | Cancer, Inflammation, IGF Signaling |
| Protein Type | Recombinant Protein |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Stability & Storage
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
