Recombinant Human IgSF11, N-terminal

Recombinant human immunoglobulin superfamily, member 11, derived from transfected human HEK293 cells.
As low as $131.00
In stock
Only %1 left
Code:
230-30108
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Product Description

Specifications

Size 10 ug, 50 ug, 200 ug
Species Human
Tag N-terminal histidine tag
Format Lyophilized powder
Formulation Lyophilized from a 0.2 µm filtered solution in PBS
Protein Name / Synonyms IgSF11, N-terminal, Immunoglobulin Superfamily, Member 11, Brain And Testis-Specific Immunoglobulin Superfamily Protein, V-Set And Immunoglobulin Domain-Containing Protein 3, Cancer/Testis Antigen 119, BT-IgSF, CXADRL1, VSIG3, V-Set And Immunoglobulin Domain Containing 3, CXADR Like 1, IgSF13, BTIGSF, IgSF11, CT119
Accession Number Q5DX21-2
Gene Symbols IGSF11
Concentration (lot specific) Determined by Pierce BCA protein assay kit
Purity >95%
Purity Determined By SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Reconstitution Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.
Expressed Region Ala20-Gly244
Expression System Human Embryonic Kidney 293 Cells
Molecular Weight Recombinant human IgSF11 protein has a calculated molecular mass of 24 kDa. Due to the abundant glycosylation, it migrates as approximately 30-35 kDa protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Purification His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
SDS-PAGE Image
SDS-PAGE
Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with Protein Deglycosylation Kit under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.
Lane 1: Protein standard ladder (kDa)
Lane 2: Untreated protein under reducing conditions
Lane 3: Treated protein with deglycosylation enzymes under native conditions
Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.
Lane 5: Deglycosylation mixture only without target proteins.

Stability & Storage

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Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.

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