Recombinant Human IL-1 beta, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 10 µg, 50 µg, 100 µg, 250 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P01584 |
| Gene Id | 3553 |
| Gene Symbols | IL1B, IL1F2 |
| Protein Name / Synonyms | Interleukin-1 beta (IL-1 beta) (Catabolin), Interleukin-1 beta, IL-1 beta, Catabolin, Interleukin 1 Beta, IL1F2, Pro-Interleukin-1-Beta, Preinterleukin 1 Beta, IL1-BETA, IL-1 |
| Expressed Region | Ala117-Ser269 |
| Expression System | HEK293 cells |
| Tag | N-terminal histidine tag |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein has a calculated molecular weight of about 17 kDa. Due to the post-translation modifications, it migrates as approximately 25 kDa protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS |
| Concentration (lot specific) | Determined by Pierce BCA protein assay kit |
| Preservative | None |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water. |
| Endotoxin Level | Not determined |
| Research Area | Angiogenesis, Inflammation |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Introduction
Interleukin-1 Beta (IL-1β) is a pro-inflammatory cytokine predominantly produced by activated macrophages. It plays a pivotal role in mediating immune and inflammatory responses by inducing the expression of various genes involved in inflammation, immunity, and hematopoiesis. IL-1β is synthesized as an inactive precursor and requires cleavage by caspase-1 to become active. Dysregulation of IL-1β production or signaling is associated with various pathological conditions, including autoimmune diseases, chronic inflammatory diseases, and sepsis.
Reference: https://pubmed.ncbi.nlm.nih.gov/8630372/
SDS-PAGE Image
Lane 1: Protein standard ladder (kDa).
Lane 2: Untreated protein under reducing conditions.
Lane 3: Treated protein with deglycosylation enzymes under native conditions.
Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.
Lane 5: Deglycosylation mixture only without target proteins.
Activity
Stimulating cell growth significantly. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The EC50 for this effect is 5.8 ng/mL.
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.
Custom Labeling Request
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