3553

Interleukin-1 beta, IL-1 beta, Catabolin, Interleukin 1 Beta, IL1F2, Pro-Interleukin-1-Beta, Preinterleukin 1 Beta, IL1-BETA, IL-1.

Unpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20,000 g centrifugation at 4°C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80°C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control.

Recombinant protein has a calculated molecular weight of about 20 kDa. The actual molecular weight may increase slightly due to the potential post-modifications (PTMs).

Lyophilized from a 0.2 µm filtered solution,in PBS containing mammalian cell protease inhibitor cocktail

None