Recombinant human IL10-RA with an N-terminal His-tag, derived from the transfected human HEK293 cells.
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| Size | 10 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | Q13651 |
| Gene Symbols | IL10RA |
| Protein Name / Synonyms | Interleukin 10 Receptor Alpha, IL10R, Interleukin-10 Receptor Subunit 1, IL-10 Receptor Subunit Alpha, IL-10R Subunit Alpha, IL-10R Subunit 1,CDW210A,IL-10R1, IL-10RA, Interleukin-10 Receptor Subunit Alpha. |
| Expressed Region | His22-Asn235 |
| Expression System | HEK293 cells |
| Tag | N-terminal histidine tag |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 25 kDa. Due to the abundant glycosylation, it migrates as approximately 40-50 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below. |
| Format | Liquid |
| Formulation | Supplied as a 0.2 µm filtered solution in PBS (pH 7.4) |
| Concentration (lot specific) | Lot specific (see the label on the vial), determined by Pierce BCA protein assay |
| Endotoxin Level | <0.5 EU per µg of the protein as determined by the LAL method |
| Research Area | Inflammation, Autophagy |
| Recombinant or Native | Recombinant |
| Shipping Type | Blue ice |
| Storage | -20°C |

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Figure 1. Deglycosylation analysis of purified recombinant proteins. Lane 1, protein ladder (kDa). Lane 2, glycosylated protein (delivered to customers). Lanes 3 and 4, deglycosylated proteins under native and denature conditions respectively.
Figure 1. Deglycosylation analysis of purified recombinant proteins. Lane 1, protein ladder (kDa). Lane 2, glycosylated protein (delivered to customers). Lanes 3 and 4, deglycosylated proteins under native and denature conditions respectively.